Toxoplasma gondii major surface antigen (SAG1): in vitro analysis of host cell binding

Previous studies have indicated that SAG1, the major surface molecule of the protozoan parasite Toxoplasma gondii, is an important attachment ligand for the host cell. However, the research data that supports this claim comes largely from studies investigating tachyzoite binding, and not SAG1 binding per se. In this study we successfully developed an in vitro attachment assay to directly evaluate the mechanism of SAG1-host cell binding. Competition experiments were then performed using SAG1 that had been pre-treated with the neoglycoprotein BSA-glucosamide or with antibody. Soluble BSA-glucosamide blocked SAG1 attachment to MDBK cells in a dose-dependent manner, implying that SAG1 binding is mediated, in part, via attachment to host cell surface glucosamine. Interestingly, pre-incubation of SAG1 in polyclonal sera from chronically infected mice failed to block binding. This challenges the assumption that anti-SAG1 antibodies block parasite attachment through the masking of SAG1 host cell binding domains. Taken together, this evidence presents new strategies for understanding SAG1-mediated attachment

Strong singularity of singular masas in II₁ factors

A singular masa A in a II1 factor N is defined by the property that any unitary w ∈ N for which A=wAw* must lie in A. A strongly singular masa A is one that satisfies the inequality ||EA- EwAw*||∞,2 ≥||w- EA(w)||2 for all unitaries w ∈ N where EA is the conditional expectation of N onto A, and ||⋅||∞,2 is defined for bounded maps Φ : N → N by sup{||Φ (x)||2:x ∈ N,||x||≤1}. Strong singularity easily implies singularity, and the main result of this paper shows the reverse implication

Psychological distress, physical illness, and risk of coronary heart disease

Study objective: The aims of this study are to confirm the association between psychological distress and coronary heart disease (CHD) using an epidemiological community study with hospital admissions data and to examine if any association is explained by existing illness. Design: Prospective cohort study modelling the association between psychological distress, measured using the 30 item general health questionnaire (GHQ), and hospital admissions data for CHD (ICD 410–414), using proportional hazards modelling adjusted for sociodemographic, CHD risk factors, and angina, bronchitis, diabetes, ischaemia, and stroke. Setting: Two suburbs of Glasgow, Renfrew and Paisley, in Scotland. Participants: 6575 men and women aged 45–64 years from Paisley. Main: results: Five year CHD risk in distressed men compared with non-distressed men was 1.78 (95% confidence intervals (CI), 1.15 to 2.75) in age adjusted analysis, 1.78 (95% CI, 1.14 to 2.79) with sociodemographic and CHD risk factor adjustment, and 1.61 (95% CI 1.02 to 2.55) with additional adjustment for existing illness. Psychological distress was unrelated to five year CHD risk in women. In further analysis, compared with healthy, non-distressed men, distressed physically ill men had a greater risk of CHD than non-distressed physically ill men, a relative risk of 4.01 (95% CI 2.42 to 6.66) compared with 2.12 (95% CI 1.35 to 3.32). Conclusion: The association of psychological distress with an increased risk of five year CHD risk in men could be a function of baseline physical illness but an effect independent of physical illness cannot be ruled out. Its presence among physically ill men greatly increases CHD risk

Letter from S.A. Smith to William Langer Regarding Mr. Sheets, February 24, 1919.

Letter dated February 24, 1919 from Sheriff S.A. Smith to William Langer regarding Mr. Sheets, whom Langer sent to investigate the election case. Smith writes that Mr. Sheets is doing a great job, adding that he thinks there is some merit to the complaints made about the election.https://commons.und.edu/langer-papers/1324/thumbnail.jp

Letter from S.A. Smith to William Langer Regarding Absent Votes for Governor Frazier, November 10, 1918.

Letter from Sheriff S.A. Smith to William Langer regarding absent votes for Governor Frazier, November 10, 1918. Smith writes that he suspects election tampering in three of his precincts. He writes that there were twenty absent votes but none for Fraizer even though three voters say they voted for him. Smith asks if there is any way to see the ballots without contesting the election. He also writes that he hopes Mrs. Langer is feeling better.https://commons.und.edu/langer-papers/1312/thumbnail.jp

Letter from S.A. Smith to William Langer Regarding Bootlegging, August 29, 1918.

Letter from Sheriff S.A. Smith to William Langer Regarding Brady, August 29, 1918. Smith writes to give a report following up on a previous conversation between he and Langer about a man named Brady, a bootlegger who Smith has arrested before. Smith writes that Brady is still getting booze from Yates and whoever transports it must be traveling by either Baker or Marmath. Smith asks if Langer would be able to set someone on Brady\u27s trail at Mandan, as Smith suspects Brady has a fresh load stashed there.https://commons.und.edu/langer-papers/1300/thumbnail.jp

Psychological distress and chronic obstructive pulmonary disease in the Renfrew and Paisley (MIDSPAN) study

Background: This study examined whether psychological distress might be a predictor of chronic obstructive pulmonary disease (COPD). Method: The relation between psychological distress at baseline, measured by the general health questionnaire (GHQ), and chronic bronchitis three years later, as measured by the Medical Research Council (MRC) bronchitis questionnaire and forced expiratory flow in one second (FEV1), was examined in 1682 men and 2203 women from the Renfrew and Paisley (MIDSPAN) study. The analyses were run on men and women separately and adjustments were made for age, socioeconomic position, and lung function at baseline (FEV1). People with chronic diseases at baseline were then excluded to give a "healthy" baseline cohort. The effect of psychological distress on individual components of the MRC bronchitis questionnaire and FEV1 was also assessed. Results: In multivariate analyses of the whole cohort baseline psychological distress in women was associated with reduced FEV1 at follow up (OR 1.31 95% CI 1.0 to 1.73) after adjustment. In women, in the healthy cohort, psychological distress was associated with chronic bronchitis (OR 2.00, 95% CI 1.16 to 3.46), symptoms of bronchial infection (OR 2.14, 95% CI 1.44 to 3.19), symptoms of breathlessness (OR 3.02, 95% CI 1.99 to 4.59), and reduced FEV1 (OR 1.62, 95% CI 1.13 to 2.32). In men psychological distress predicted symptoms of bronchial infection (OR 2.09, 95% CI 1.28 to 3.42). Conclusion: This study supports research suggesting that psychological distress is associated with COPD and shows that psychological distress predicts COPD in women. The robustness of the association and the exact mechanism requires further investigation

An evaluation of response scale formats of the culture assessment instrument.

The aim of the study was to investigate which response scale, of the four, five, or six-point response scales would yield the best metric properties on the Culture Assessment Instrument. This was achieved by utilising data sets where the respective scales were used. The subjects included participants of various organisations, ages, educational levels, language and gender groups. No one scale could be identified as having the better metric properties. The lack of conclusive results is attributed to differences in education, aggregation effect, possibility of different units of measurement being measured and the manner in which Cronbach Alpha is calculated

So many genes, so little time: A practical approach to divergence-time estimation in the genomic era

Phylogenomic datasets have been successfully used to address questions involving evolutionary relationships, patterns of genome structure, signatures of selection, and gene and genome duplications. However, despite the recent explosion in genomic and transcriptomic data, the utility of these data sources for efficient divergence-time inference remains unexamined. Phylogenomic datasets pose two distinct problems for divergence-time estimation: (i) the volume of data makes inference of the entire dataset intractable, and (ii) the extent of underlying topological and rate heterogeneity across genes makes model mis-specification a real concern. “Gene shopping”, wherein a phylogenomic dataset is winnowed to a set of genes with desirable properties, represents an alternative approach that holds promise in alleviating these issues. We implemented an approach for phylogenomic datasets (available in SortaDate) that filters genes by three criteria: (i) clock-likeness, (ii) reasonable tree length (i.e., discernible information content), and (iii) least topological conflict with a focal species tree (presumed to have already been inferred). Such a winnowing procedure ensures that errors associated with model (both clock and topology) mis-specification are minimized, therefore reducing error in divergence-time estimation. We demonstrated the efficacy of this approach through simulation and applied it to published animal (Aves, Diplopoda, and Hymenoptera) and plant (carnivorous Caryophyllales, broad Caryophyllales, and Vitales) phylogenomic datasets. By quantifying rate heterogeneity across both genes and lineages we found that every empirical dataset examined included genes with clock-like, or nearly clock-like, behavior. Moreover, many datasets had genes that were clock-like, exhibited reasonable evolutionary rates, and were mostly compatible with the species tree. We identified overlap in age estimates when analyzing these filtered genes under strict clock and uncorrelated lognormal (UCLN) models. However, this overlap was often due to imprecise estimates from the UCLN model. We find that “gene shopping” can be an efficient approach to divergence-time inference for phylogenomic datasets that may otherwise be characterized by extensive gene tree heterogeneity